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Recombinant and wild‐type Pseudomonas aureofaciens strains introduced into soil microcosms: effect on decomposition of cellulose and straw
Author(s) -
ENGLAND L. S.,
LEE HUNG,
TREVORS J. T.
Publication year - 1995
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.1995.tb00211.x
Subject(s) - biology , microcosm , incubation , inoculation , straw , cellulose , microbiology and biotechnology , pseudomonas , zoology , veterinary medicine , horticulture , agronomy , bacteria , ecology , biochemistry , genetics , medicine
The effect of a genetically engineered Pseudomonas aureofaciens (Ps3732RNL11) strain (GEM) and the parental wild‐type (Ps3732RN) on decomposition of cellulose paper, straw and calico cloth was assessed after 18 weeks incubation in laboratory soil microcosms. Effect(s) of inoculum density (10 3 , 10 5 , and 10 8 cells/ g dry soil) and single versus multiple bacterial inoculations were also investigated. Cellulose paper was completely decomposed after 18 weeks in all treatments. There were no significant differences (95% level), between treatments, in percentage decomposition of either straw or calico cloth. Recovery of the GEM at 18 weeks, using viable plating, was limited to treatments originally receiving 10 8 cells/g dry soil. Log 1.8 CFU/g dry soil were recovered from the single dose treatment while log 4.2 CFU/g dry soil were recovered from the multiple dose treatment Biolog metabolic tests were used to determine if the GEM or parental wild‐type had any effect on overall carbon utilization in soil. Results suggested they did not. Detection of the recombinant lac ZY gene sequence in soil using PCR suggested the possibility of viable but nonculturable cells and/or persistence of chromosomal DNA.