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DNA fingerprinting reveals significant amounts of genetic variation in a wild raspberry Rubus idaeus population
Author(s) -
ANTONIUS K.,
NYBOM H.
Publication year - 1994
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.1994.tb00119.x
Subject(s) - rubus , biology , blowing a raspberry , apomixis , dna profiling , population , natural population growth , botany , microsatellite , dna , genetics , horticulture , ploidy , gene , allele , demography , sociology
Establishing the genotypic distribution in natural plant populations is an important part of ecological studies concerning plant growth, reproduction and turn‐over. Restriction enzyme‐digested DNA samples, isolated from 24 plants of a natural Rubus idaeus population, were analysed with DNA fingerprinting using the M13 repeat sequence as well as a synthetic (AC)/(TG) polydinucleotide as hybridization probes. All the examined samples exhibit unique DNA fingerprint patterns, suggesting that vegetative reproduction may be considerably more restricted in wild R. idaeus populations than previously assumed. By comparison, all samples of the apomictic blackberry species Rubus nessensis , collected on the same location, were completely identical.