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Identification of hypervariable single locus minisatellite DNA probes in the blue tit Parus caeruleus
Author(s) -
VERHEYEN G. R.,
KEMPENAERS B.,
BURKE T.,
BROECK M. VAN DEN,
BROECKHOVEN C. VAN,
DHONDT A.
Publication year - 1994
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.1994.tb00114.x
Subject(s) - minisatellite , biology , genetics , locus (genetics) , cosmid , loss of heterozygosity , microsatellite , genomic library , dna , population , dna profiling , allele , gene , base sequence , demography , sociology
We report the isolation of a set of hypervariable minisatellite DNA sequences from a blue tit Parus caeruleus genomic DNA library. In our strategy, we cloned a minisatellite‐rich DNA fraction into a charomid vector. The resulting cosmid library was screened with the two minisatellite DNA probes 33.6 and 33.15 for recombinants containing a minisatellite DNA insert. A total of 233 positive clones were isolated. Of 37 clones that have been analysed, nine gave polymorphic signals and can be used as single locus probes (SLPs). Four of the SLPs were investigated in more detail. The number of alleles, the heterozygosity and the mutation rate were estimated. Linkage analysis revealed that two of these loci were linked. The SLPs are of value to studies of the mating system and reproductive success in the blue tit, and may also be useful in population genetic studies.