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Segregation analysis of random amplified polymorphic DNA (RAPD) markers in Picea abies Karst.
Author(s) -
BUCCI G.,
MENOZZI P.
Publication year - 1993
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.1993.tb00012.x
Subject(s) - rapd , biology , mendelian inheritance , picea abies , genetics , genetic marker , population , botany , genetic diversity , gene , demography , sociology
Abstract The reliability of arbitrarily primed amplification products was tested. The segregation analysis of 266 amplification products obtained using 17 different 10‐mer oligonucleotides in 34 megagametophytes from a single tree of Picea abies was carried out. Fifty‐four out of the 165 variable bands fit the 1:1 segregation ratio expected for Mendelian traits. The segregation ratio of a subset of six RAPD markers in five other individuals from the same population confirmed their genetic nature. Our results strengthen the evidence previously reported that RAPDs markers can be considered Mendelian traits useful in the detection of genetic variability among both different individuals and populations.