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Screening techniques for detecting allelic variation in DNA sequences
Author(s) -
LESSA E. P.,
APPLEBAUM G.
Publication year - 1993
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.1993.tb00006.x
Subject(s) - heteroduplex , biology , temperature gradient gel electrophoresis , single strand conformation polymorphism , genetics , dna , computational biology , allele , gel electrophoresis , polymorphism (computer science) , polymerase chain reaction , microbiology and biotechnology , gene , 16s ribosomal rna
This article reviews four ‘DNA screening techniques’, namely heteroduplex analysis, single‐strand conformational polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) as tools for the study of allelic variation in natural populations. The resolving power, advantages, and limitations of each technique are discussed and compared. We also provide some criteria for choosing among techniques and illustrate some practical issues with examples taken primarily from our own laboratory experience.