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Survival and detection of an introduced Escherichia coli containing the porcine somatotropin gene from a waste treatment microcosm
Author(s) -
GEALT M. D.,
SELVARATNAM S.,
ABRAMS L.,
SYNENKI R. M.
Publication year - 1992
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.1992.tb00179.x
Subject(s) - biology , escherichia coli , microcosm , microbiology and biotechnology , plasmid , bacteria , gene , pbr322 , enterobacteriaceae , genetics , ecology
The number Escherichia coli containing the porcine somatotropin (pST) gene cloned into plasmid pBR322 recovered from a laboratory‐scale waste treatment microcosm decreased over a period of several days following their introduction. The pST gene was detected by hybridization analysis in these bacteria when recovered from authentic wastewater at 72 h after introduction, but the gene was not detected in bacteria recovered at 144 h. No significant difference in recovery, as judged by auxotrophic and antibiotic‐resistance markers, was noted between the pST‐containing strain and an isogenic E. coli without pST , indicating that presence of the pST gene confers no survival advantage or disadvantage on the bacterial host.