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DNA stabilization and amplification from museum collections of extracts originally intended for allozyme analysis
Author(s) -
TAN ANMING,
ORREGO CRISTIÁN
Publication year - 1992
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.1992.tb00176.x
Subject(s) - biology , polymerase chain reaction , dna , isozyme , genetics , microbiology and biotechnology , gene , biochemistry , enzyme
Protein extracts originally prepared for isozyme electrophoresis two decades ago contain surviving DNA sequences susceptible to amplification by the polymerase chain reaction (PCR). Amplification was also possible after stabilization of such extracts on filter paper and immersion under mineral oil without refrigeration.