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Detection of Salmonella sp. in Dermanyssus gallinae using an FTA ® filter‐based polymerase chain reaction
Author(s) -
VALIENTE MORO C.,
DESLOIRE S.,
CHAUVE C.,
ZENNER L.
Publication year - 2007
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/j.1365-2915.2007.00684.x
Subject(s) - biology , salmonella , polymerase chain reaction , mite , acari , flock , salmonella enteritidis , microbiology and biotechnology , veterinary medicine , bacteria , 16s ribosomal rna , vector (molecular biology) , virology , zoology , ecology , genetics , gene , medicine , recombinant dna
Salmonella spp. bacteria are responsible for some of the most important zoonoses worldwide. Because Dermanyssus gallinae (DeGeer) (Acari: Dermanyssidae) has been recently reported to be an experimental vector of Salmonella Enteritidis, it would be of benefit to evaluate the presence of this bacterium in mites. A molecular detection tool associating a simple filter‐based DNA preparation with a specific 16S rDNA Salmonella sp. polymerase chain reaction (PCR) amplification was described. The limit of detection with this method was 2 × 10 4 bacteria per mite. To adapt this technique for large‐scale studies, two sizes of mite pools were tested and a preliminary investigation was carried out on mites from 16 currently or previously contaminated farms. Mites sampled from one farm of each type were positive for Salmonella , suggesting that Dermanyssus could act as a reservoir between flocks. In further investigations, it will be necessary to carry out a large‐scale study to assess the role of D. gallinae in the epidemiology of avian salmonellosis.