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Antibacterial substances of low molecular weight isolated from the blowfly, Lucilia sericata
Author(s) -
HUBERMAN L.,
GOLLOP N.,
MUMCUOGLU K. Y.,
BREUER E.,
BHUSARE S. R.,
SHAI Y.,
GALUN R.
Publication year - 2007
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/j.1365-2915.2007.00668.x
Subject(s) - micrococcus luteus , lucilia , biology , calliphoridae , lysis , micrococcus , molecular mass , antibacterial activity , bacteria , proline , microbiology and biotechnology , biochemistry , amino acid , staphylococcus aureus , botany , larva , enzyme , genetics
Low molecular weight compounds were isolated by high‐performance liquid chromatography from the maggot or haemolymph extracts of Lucilia sericata (Meigen) (Diptera: Calliphoridae). Using gas chromatography‐mass spectrometry analysis, three compounds were obtained: p ‐hydroxybenzoic acid (molecular weight 138 Da), p ‐hydroxyphenylacetic acid (molecular weight 152 Da) and octahydro‐dipyrrolo[1,2‐a;1′,2′‐d] pyrazine‐5,10‐dione (molecular weight 194 Da), also known as the cyclic dimer of proline (or proline diketopiperazine or cyclo[Pro,Pro]). All three molecules revealed antibacterial activity when tested against Micrococcus luteus and/or Pseudomonas aeruginosa , and the effect was even more pronounced when these molecules were tested in combination and caused lysis of these bacteria.