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Identifying Canadian mosquito species through DNA barcodes
Author(s) -
CYWINSKA A.,
HUNTER F. F.,
HEBERT P. D. N.
Publication year - 2006
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/j.1365-2915.2006.00653.x
Subject(s) - biology , dna barcoding , range (aeronautics) , mitochondrial dna , zoology , barcode , ecology , species complex , phylogenetic tree , genetics , materials science , computer science , composite material , gene , operating system
A short fragment of mt DNA from the cytochrome c oxidase 1 (CO1) region was used to provide the first CO1 barcodes for 37 species of Canadian mosquitoes (Diptera: Culicidae) from the provinces Ontario and New Brunswick. Sequence variation was analysed in a 617‐bp fragment from the 5′ end of the CO1 region. Sequences of each mosquito species formed barcode clusters with tight cohesion that were usually clearly distinct from those of allied species. CO1 sequence divergences were, on average, nearly 20 times higher for congeneric species than for members of a species; divergences between congeneric species averaged 10.4% (range 0.2–17.2%), whereas those for conspecific individuals averaged 0.5% (range 0.0–3.9%).