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Effects of temperature on virogenesis of bluetongue virus serotype 11 in Culicoides variipennis sonorensis
Author(s) -
MULLENS BRADLEY A.,
TABACHNICK WALTER J.,
HOLBROOK FREDERICK R.,
THOMPSON LEE H.
Publication year - 1995
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/j.1365-2915.1995.tb00119.x
Subject(s) - biology , orbivirus , culicoides , serotype , virology , ceratopogonidae , virus , zoology , reoviridae , rotavirus
Abstract.Culicoides variipennis sonorensis females were fed bluetongue virus serotype 11 mixed in sheep blood and were held at constant temperatures of 32, 27, 21 and 15 o C. Virogenesis, as measured by enzyme‐linked immunosorbent assay (ELISA), proceeded significantly faster at higher temperatures. Based on ELISA absorbance ≥0.2, some flies first were categorized as infected after 1 day, 2 days and 4 days at 32, 27 and 21 o C, respectively. Peak levels of virus antigen were seen after 5–7, 7–13 and 18–22 days for flies held at 32, 27 and 21 o C, respectively. There was no significant virus replication in flies held at 15 o C for 22 days, but latent virus replicated and was detected easily (44% infection) 4–10 days after these flies were transferred to 27 o C. The implications for temperature effects on bluetongue epizootiology are discussed.