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DNA probes for the identification of members of the Simulium damnosum complex (Diptera: Simuliidae)
Author(s) -
POST R. J.,
FLOOK P.
Publication year - 1992
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/j.1365-2915.1992.tb00637.x
Subject(s) - biology , sibling species , simulium , intraspecific competition , identification (biology) , genetics , larva , evolutionary biology , zoology , ecology
Genomic libraries in plasmid have been constructed from various sibling species of blackflies of the Simulium damnosum complex from West Africa. Three cloned repetitive sequences, which show variation in copy number between sibling species, have been isolated. These clones can be used as probes for the dot‐blot identification of larvae, pupae or adults into the three main West African subcomplexes, i.e. damnosum, squamosum and sanctipauli subcom‐plexes. The sequences also show some intraspecific variation in copy number.