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Oral susceptibility of Aedes albopictus to dengue type 2 virus: a study of infection kinetics, using the polymerase chain reaction for viral detection
Author(s) -
TARDIEUX ISABELLE,
POUPEL OLIVIER,
RODHAIN FRANCOIS,
LAPCHIN LAURENT
Publication year - 1992
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/j.1365-2915.1992.tb00626.x
Subject(s) - biology , aedes albopictus , virology , virus , polymerase chain reaction , dengue fever , aedes aegypti , dengue virus , aedes , gene , genetics , larva , botany
Female Aedes albopictus mosquitoes, aged 1 week, were infected with DEN‐2 dengue virus. The kinetics of infection in mosquito brain and mesenteron were monitored using DNA probes with polymerase chain reaction (PCR) amplification of target DNA sequences coding for DEN‐2 virus envelope protein, compared with the standard immunofluorescence assay technique (IFA). Rates of virus detection in the mesenteron of orally infected mosquitoes rose to 38% by day 4 post‐inoculation, then declined until day 8, followed by irregular peaks around days 11–14 and subsequently. In mosquito head squashes, virus was detected from day 4 onwards, reaching 38% positive by day 18. Salivary glands of all the same females were found to be positive for virus by day 8 onwards. Parenterally infected Ae.albopictus females were all positive for DEN‐2 in the brain and salivary glands 8 days post‐inoculation. In every case, results obtained with the PCR matched those from the IFA. Our DNA probe with PCR procedure can therefore be utilized as a sensitive and reliable method for studies of DEN‐2 vectors.