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Testing Anopheles albimanus for genetic linkage of insecticide resistance genes by combining insecticide bioassay and biochemical methods
Author(s) -
LINES J. D.,
FfRENCHCONSTANT R. H.,
KASIM S. H.
Publication year - 1990
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/j.1365-2915.1990.tb00464.x
Subject(s) - biology , propoxur , bioassay , genetics , anopheles albimanus , dieldrin , backcrossing , pesticide resistance , allele , gene , insecticide resistance , toxicology , pesticide , anopheles , malaria , agronomy , immunology
. A microtitre‐plate assay which distinguishes propoxur‐resistant from susceptible Anopheles albimanus Weidemann was used to test for linkage between the genes for propoxur‐ and dieldrin‐resistance. The adult progeny of a backcross between a doubly‐resistant colony and a fully susceptible colony were exposed in conventional test kits to the standard discriminating dose of dieldrin, and kept in the insectary overnight. Both live and dead insects were then assayed individually for propoxur‐resistance. The results showed that heterozygotes for propoxur‐resistance could be reliably distinguished from susceptibles whether or not they had been killed up to 24 h previously by dieldrin treatment. In this way all the backcross progeny could be scored at both resistance loci, and all four genotypic classes identified. Resistant and susceptible alleles at the two loci were inherited independently, demonstrating the absence of linkage. The usual method of testing for linkage between resistance genes is inefficient and open to bias, because insects have to be exposed to each insecticide in turn, and only half of them can be scored at both loci. The method shown here avoids these drawbacks.