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Early detection of cattle grub (Hypoderma lineatum and H.bovis) (Diptera, Oestridae) using ELISA
Author(s) -
COLWELL D. D.,
BARON R. W.
Publication year - 1990
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/j.1365-2915.1990.tb00257.x
Subject(s) - biology , infestation , veterinary medicine , myiasis , larva , zoology , parasite hosting , ecology , agronomy , medicine , world wide web , computer science
. .An enzyme‐linked immunosorbent assay (ELISA) to detect cattle grub‐infested animals in the autumn in Alberta has been developed. Antibody to Hypoderma lineatum de Vill. was detectable as early as 6 weeks post‐infestation in artificially infested steers. Peak antibody concentrations preceded the peak in maximum ‘apparent’ grub numbers which occurred between 37 and 43 weeks after infestation. Natural infestations with H. lineatum and H. bovis L., ranging from one to ninety‐two grubs per calf, were readily detected by November and the incidence of false positives in uninfested calves was only 5%. Low level (1–4 grubs) infestations of H. bovis only became detectable in February with peak antibody concentrations occurring at the end of April. Prevalence of cattle grub infestations in southern Alberta was shown to be 37% during this study.