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Genetic transformation of the mosquito Aedes aegypti by micro‐injection of DNA
Author(s) -
MORRIS ALISON C.,
EGGLESTON PAUL,
CRAMPTON JULIAN M.
Publication year - 1989
Publication title -
medical and veterinary entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 82
eISSN - 1365-2915
pISSN - 0269-283X
DOI - 10.1111/j.1365-2915.1989.tb00467.x
Subject(s) - aedes aegypti , biology , plasmid , transformation (genetics) , selectable marker , transposable element , genetics , dna , drosophila melanogaster , aedes , electroporation , genome , larva , virology , gene , dengue fever , ecology
. We report the successful introduction of heterologous DNA sequences into embryos of the mosquito Aedes aegypti (L.) by microinjection. The injected DNA carrried P transposable element sequences, derived from and known to facilitate transformation in Drosophila melanogaster. Two plasmids, one of which carried a dominant selectable marker, were introduced into the posterior of embryos prior to pole cell formation and subsequently taken up into the germ line of transformed individuals. Stable transfer of the selectable marker (G418 resistance) was demonstrated over two generations. The precise nature of these putative P mediated integration events is currently being investigated. However, the results presented here establish the technique of DNA transformation for the genetic manipulation of Aedes aegypti.