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Preactivation of the interferon signalling in liver is correlated with nonresponse to interferon alpha therapy in patients chronically infected with hepatitis B virus
Author(s) -
Xiao C.,
Qin B.,
Chen L.,
Liu H.,
Zhu Y.,
Lu X.
Publication year - 2012
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/j.1365-2893.2011.01471.x
Subject(s) - interferon , gene , alpha interferon , immune system , biology , immunology , gene expression profiling , gene expression , hepatitis b virus , virus , cancer research , medicine , genetics
Summary. Interferon alpha (IFN‐α) therapy is widely used to treat patients with chronic hepatitis B (CHB) but the sustained response rate is low, and the molecular mechanisms for the ineffectiveness of IFN‐α treatments are not known. We screened differentially expressed genes between responders (Rs) and nonresponders (NRs) in patients with CHB treated with IFN‐α to explore the molecular basis for treatment failure. Expression profiling was performed on percutaneous needle liver biopsy specimens taken before therapy. Gene expression levels were compared between seven patients who did not respond to therapy (NR) and six who did respond (R). Gene ontology category and KEGG pathway were analysed for differentially expressed genes, and the selected differentially expressed genes were confirmed using real‐time polymerase chain reaction. We identified 3592 genes whose expression levels differed significantly between all Rs and NRs ( P < 0.05); many of these genes are IFN‐stimulated genes (ISGs) and immune‐related genes. The ISGs were more highly expressed, while immune‐related genes were inhibited in NRs before IFN‐α treatment. Two ISGs (CEB1 and USP18) that are linked in an IFN inhibitory pathway are highly expressed in NRs, and a potential antiviral gene ISG20 was inhibited in NRs, suggesting a possible rationale for treatment nonresponse. Patients who do or do not respond to IFN have different liver gene expression profiles before IFN‐α treatment. Preactivation of the IFN signalling pathway leading to the increased expression of inhibitory ISGs and inhibition of immune response in the pretreatment livers was associated with treatment failure.