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Detection of hepatitis B virus markers using a biosensor based on imaging ellipsometry
Author(s) -
Qi C.,
Zhu W.,
Niu Y.,
Zhang H. G.,
Zhu G. Y.,
Meng Y. H.,
Chen S.,
Jin G.
Publication year - 2009
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/j.1365-2893.2009.01123.x
Subject(s) - hbsag , hepatitis b virus , antibody , virology , antigen , microbiology and biotechnology , hepatitis b , receiver operating characteristic , ligand (biochemistry) , biosensor , chemistry , biology , medicine , virus , immunology , biochemistry , receptor
Summary. A biosensor based on imaging ellipsometry (BIE) has been developed and validated in 169 patients for detecting five markers of hepatitis B virus (HBV) infection. The methodology has been established to pave the way for clinical diagnosis, including ligand screening, determination of the sensitivity, set‐up of cut‐off values (CoVs) and comparison with other clinical methods. A matrix assay method was established for ligand screening. The CoVs of HBV markers were derived with the help of receiver operating characteristic curves. Enzyme‐linked immunosorbent assay (ELISA) was the reference method. Ligands with high bioactivity were selected and sensitivities of 1 ng/mL and 1 IU/mL for hepatitis B surface antigen (HBsAg) and surface antibody (anti‐HBs) were obtained respectively. The CoVs of HBsAg, anti‐HBs, hepatitis B e antigen, hepatitis B e antibody and core antibody were as follows: 15%, 18%, 15%, 20% and 15%, respectively, which were the percentages over the values of corresponding ligand controls. BIE can simultaneously detect up to five markers within 1 h with results in acceptable agreement with ELISA, and thus shows a potential for diagnosing hepatitis B with high throughput.