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Detection of anti‐hepatitis C virus using chemiluminescence
Author(s) -
Ornopia G. L.,
Kliramoto K.
Publication year - 1995
Publication title -
journal of viral hepatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 100
eISSN - 1365-2893
pISSN - 1352-0504
DOI - 10.1111/j.1365-2893.1995.tb00032.x
Subject(s) - chemiluminescence , immunoassay , hepatitis c virus , virology , hepatitis c , antibody , medicine , volunteer , chemistry , virus , immunology , chromatography , biology , agronomy
Summary. To determine if chemiluminescence can be used in an enzyme immunoassay (EIA) format to test for hepatitis C virus (HCV) antibody and to compare sensitivity and specificity of chemiluminescence to a licensed anti‐HCV EIA method, random volunteer donor samples were evaluated. One thousand and seventy‐four random volunteer donor samples were collected during a 2‐week period. Two aliquots of each sample were tested for anti‐HCV. One aliquot was tested using a licensed anti‐HCV 2.0 EIA test. The second aliquot was tested using the research anti‐HCV chemiluminescence assay. Confirmatory testing was done using Recombinant Immuno Blot Assay (RIBA) 2.0 HCV and HCV RNA. Of the 1074 samples, eight were found to be reactive for anti‐HCV. Seven were positive by the anti‐HCV 2.0 EIA and the chemiluminescence system. The eighth sample was chemiluminescence reactive, but EIA negative; when tested by an unlicensed EIA 3.0 HCV test, RIBA 3.0 HCV test and an HCV‐RNA assay, the sample was still negative. The same sample was also tested by RIBA 2.0 HCV and showed a reactive band to SOD. The chemiluminescence assay has a 100% sensitivity and 99.9% specificity compared to EIA and can be used as an alternative to EIA for detecting antibodies to HCV.