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Effects of flunixin meglumine and ketoprofen on mediator production in ex vivo and in vitro models of inflammation in healthy dairy cows
Author(s) -
DONALISIO C.,
BARBERO R.,
CUNIBERTI B.,
VERCELLI C.,
CASALONE M.,
RE G
Publication year - 2013
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.2012.01396.x
Subject(s) - ketoprofen , ex vivo , pharmacology , chemistry , in vivo , prostaglandin e2 , cytokine , dairy cattle , thromboxane b2 , tumor necrosis factor alpha , in vitro , medicine , immunology , biochemistry , biology , zoology , platelet , microbiology and biotechnology
Donalisio, C., Barbero, R., Cuniberti, B., Vercelli, C., Casalone, M., Re, G. Effects of flunixin meglumine and ketoprofen on mediator production in ex vivo and in vitro models of inflammation in healthy dairy cows. J. vet. Pharmacol. Therap. 36 , 130–139. In this study, ex vivo assays were carried out in dairy cows to evaluate the anti‐inflammatory effects of two nonsteroidal anti‐inflammatory drugs: ketoprofen (KETO) and flunixin meglumine (FM). Twelve healthy Holstein dairy cattle were randomly allocated to two groups ( n =6): group 1 received FM and group 2 received KETO at recommended therapeutic dosages. The anti‐inflammatory effects of both drugs were determined by measuring the production of coagulation‐induced thromboxane B 2 (TXB 2 ), lipopolysaccharides (LPS) (10 μg/mL)‐induced prostaglandin E 2 (PGE 2 ), and calcium ionophore (60 μ m )‐induced leukotrien B 4 (LTB 4 ). Cytokine production was assessed by measuring tumor necrosis factor‐α (TNF‐α), interferon‐γ (IFN‐γ) and interleukin‐8 (CXCL8) concentrations after incubation in the presence of 10 μg/mL LPS. The IC 50 of FM and KETO was determined in vitro by determining the concentration of TXB 2 and PGE 2 in the presence of scalar drug concentrations (10 −9 –10 −3 m ). Both FM and KETO inhibited the two COX isoforms in vitro, but showed a preference for COX‐1. FM and KETO showed similar anti‐inflammatory effects in the cow.