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Molecular and functional identification of organic anion transporter isoforms in cultured bovine mammary epithelial cells (BME‐UV)
Author(s) -
ALBATAINEH M. M.,
Van Der MERWE D.,
SCHULTZ B. D.,
GEHRING R.
Publication year - 2012
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.2011.01309.x
Subject(s) - organic anion transporter 1 , gene isoform , probenecid , transporter , chemistry , biochemistry , epithelial polarity , solute carrier family , microbiology and biotechnology , biology , membrane , pharmacology , gene
Al‐Bataineh, M. M., van der Merwe, D., Schultz, B. D., Gehring, R. Molecular and functional identification of organic anion transporter isoforms in cultured bovine mammary epithelial cells (BME‐UV). J. vet. Pharmacol. Therap. 35 , 209–215. Mammary epithelial cells express a diversity of membrane transporters including members of organic cation and organic anion (OAT) transporter subfamilies. Four mammal OAT isoforms have been identified: OAT‐1, OAT‐2, OAT‐3, and OAT‐4. The pharmacological significance of OAT isoforms has been emphasized because of their role in the movement of a wide variety of substrates across epithelial barriers. The present study identified (molecularly and functionally) bovine OAT isoforms in bovine mammary epithelial (BME‐UV) cells. mRNA expression levels of all tested transporters in BME‐UV cells were less than expression levels of the corresponding transporters in bovine kidney. Directionality in the flux of P ‐aminohippuric acid and acetylsalicylate, compounds known to interact with OAT‐1 and OAT‐2, respectively, across BME‐UV monolayers was not observed at the concentrations used in this study. Directionality was, however, observed in the flux of estrone sulfate (EsS). Adding probenecid, penicillin G or nonradiolabeled EsS to the apical donor compartment significantly increased the apical‐to‐basolateral flux of EsS across the BME‐UV monolayer. These results suggest that BME‐UV cells express an organic anion transport system, making it a potentially useful model to study the role of this transport system in the mammary epithelial barrier.