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Age‐related differences in prostaglandin E 2 synthesis by equine cartilage explants and synoviocytes
Author(s) -
BRISTON L.,
DUDHIA J.,
LEES P.
Publication year - 2010
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.2009.01131.x
Subject(s) - prostaglandin e2 , lipopolysaccharide , chemistry , endocrinology , osteoarthritis , medicine , cartilage , explant culture , stimulation , prostaglandin e , prostaglandin , in vitro , anatomy , pathology , biochemistry , alternative medicine
Briston, L., Dudhia, J., Lees, P. Age‐related differences in prostaglandin E 2 synthesis by equine cartilage explants and synoviocytes. J. vet. Pharmacol. Therap. 33 , 268–276. Time‐ and concentration‐related actions of lipopolysaccharide (LPS) on the synthesis of prostaglandin E 2 (PGE 2 ) were investigated in cartilage explants and synoviocytes harvested from 3 age groups of horses, all with clinically normal joint function: group A <10 years; group B 11–20 years and group C >20 years. Cartilage explants from group A horses were least and those from group C were most sensitive to LPS. Significant increases in PGE 2 concentration ( P ≤ 0.01) were obtained in group C horses in response to LPS concentrations of 1.0 μg/mL (and higher) after exposure for 24, 36 and 48 h, whereas explants from group A horses failed to respond to LPS at concentrations up to 100 μg/mL after exposure times up to 48 h. In contrast, synoviocytes from group A horses were most and those from group C horses were least sensitive to LPS stimulation. Synoviocytes from group A horses responded to LPS concentrations of 1 μg/mL (and higher) with significantly increased concentrations of PGE 2 at 24 and 36 h. Significant but numerically smaller increases in PGE 2 concentration were induced by LPS in synoviocytes from groups B and C. As the effects of high PGE 2 concentrations are catabolic for cartilage, these observations suggest that both synoviocytes and chondrocytes might exert roles in the degenerative changes which occur in cartilage in horses with osteoarthritis.