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Enrofloxacin assay validation and pharmacokinetics following a single oral dose in chickens
Author(s) -
DA SILVA R. G.,
REYES F. G. R.,
SARTORI J. R.,
RATH S.
Publication year - 2006
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.2006.00755.x
Subject(s) - enrofloxacin , chromatography , pharmacokinetics , chemistry , high performance liquid chromatography , detection limit , protein precipitation , ciprofloxacin , pharmacology , medicine , antibiotics , biochemistry
The pharmacokinetics of enrofloxacin (ENRO), a fluoroquinolone antimicrobial agent, was studied in male broiler chickens (Cobb) after single oral administration of 10 mg of ENRO/kg b.w. A high‐performance liquid chromatography‐photodiode array detector (DAD) (HPLC‐DAD) method was developed and validated and used for quantitation of ENRO and its major metabolite ciprofloxacin in plasma. The HPLC analyses were carried out using a cationic‐octadecyl mixed column and 0.05 mol/L phosphate buffer (pH 2.5)/acetonitrile as mobile phase. The sample preparation of plasma consisted of the precipitation of proteins followed by solid phase extraction on cationic‐octadecyl mixed cartridges. The method was validated considering linear range, linearity, selectivity, sensitivity, limit of detection (LOD), limit of quantitation (LOQ), intra‐ and inter‐day precisions and accuracy. The LOD and LOQ for both fluoroquinolones were 60 and 200 ng/mL for plasma. The plasma concentration vs. time graph was characteristic of a two‐compartment open model. The maximal plasma concentration of 1.5 ± 0.2 mg/mL was achieved at 9 ± 2 h. The elimination half‐life and the mean residence time of ENRO were 1.5 ± 0.2 and 15.64 h, respectively. The area under the concentration–time curve was calculated as 35 ± 4 mg·h/mL.