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Characterization of a sterile soft‐tissue inflammation model in Thoroughbred horses
Author(s) -
GUTHRIE A. J.,
SHORT C. R.,
SWAN G. E.,
MÜLDERS M.S.G.,
KILLEEN V. M.,
NURTON J. P.
Publication year - 1996
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1996.tb00007.x
Subject(s) - inflammation , tissue fluid , carrageenan , chemistry , albumin , muscle tissue , immune system , pathology , immunology , medicine , anatomy , biochemistry
This paper describes the use of subcutaneously‐placed tissue chambers as a sterile soft‐tissue inflammation model in Thoroughbred horses. Acute, nonimmune inflammation was initiated by injecting a sterile lambda carrageenan solution into a tissue chamber. This model was used to study the temporal changes in oxygen and carbon dioxide tensions, pH, bicarbonate, protein, albumin, prostaglandin E 2 (PGE 2 ) and leukotriene B 4 (LTB 4 ) concentrations, cell counts and differential counts in tissue fluid from inflamed tissue chambers and control chambers. Skin temperatures over control and inflamed chambers were also compared. Carrageenan‐induced inflammation resulted in significant increases in tissue‐fluid carbon dioxide tension, leucocyte count, albumin, and PGE 2 and LTB 4 concentrations. It also resulted in a significant decrease in tissue fluid pH and HCO 3 ‐ concentration. Inflammation did not result in significant changes in tissue‐fluid protein concentration, differential cell counts or skin temperature over the chambers. The use of this type of tissue chamber is wellsuited for studying the pathophysiology of a self‐contained, non‐immune inflammatory process. The model described in this paper could prove to be very useful in studies of the distribution of anti‐inflammatory drugs and the effects of such drugs on various aspects of the inflammatory process.