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Radioreceptor assay for determination of xylazine and medetomidine in sheep plasma
Author(s) -
MUGE D.K.,
CHAMBERS J.P.,
LIVINGSTON A.
Publication year - 1995
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1995.tb00546.x
Subject(s) - medetomidine , xylazine , chemistry , chromatography , anesthesia , medicine , ketamine , heart rate , blood pressure
A radioreceptor assay technique is described for the measurement of xylazine and medetomidine in sheep plasma. The assay was based on the displacement of tritiated clonidine from a 2‐adrenoceptors in a rat brain homogenate by xylazine or medetomidine extracted from plasma. Plasma samples from sheep which had been given xylazine and medetomidine were treated with alumina to remove endogenous catecholamines which would otherwise have bound to α 2 ‐ ‐ adrenoceptors and interfered with the assay. The drugs were then extracted using chloroform, reconstituted in buffer and used to displace [ 3 H]clonidine. The concentration of α 2 ‐agonist was calculated by reference to standard curves. The method had a detection limit of 2.5 ng/mL for xylazine and 0.24 ng/mL for medetomidine. The assay could also be used to detect metabolites capable of binding to α 2 ‐receptors.