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An image analysis system: an objective and accurate alternative for reading the agar diffusion test
Author(s) -
SCHOEVERS E. J.,
TERLOU M.,
PIJPERS A.,
VERHEIJDEN J. H. M.
Publication year - 1994
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1994.tb00519.x
Subject(s) - agar , diffusion , coefficient of variation , agar diffusion test , chromatography , urine , bioassay , chemistry , biology , biochemistry , physics , escherichia coli , bacteria , gene , thermodynamics , genetics
Schoevers, E.J., Terlou, M., Pijpers, A., Verheijden, J.H.M. An image analysis system: an objective and accurate alternative for reading the agar diffusion test. J. vet. Pharmacol Therap. 17, 38–42. A computerized image analysis system (IAS) has been used to develop a new method for reading the agar diffusion test automatically. In four experiments a total of 88 porcine plasma and 95 urine samples were screened for oxytetra‐cycline by the agar diffusion test. The inhibition zones were measured by hand and by the IAS directly from the bioassay plate and by the IAS from the photonegative taken from the plate. Both methods were positively correlated with the hand method for plasma (0.9716, 0.9669) and urine (0.9878, 0.9731) in the range tested for 0.1 to 2.0 (m̈/ml. Moreover, the coefficient of variation and the day‐to‐day‐variation amounted to 1,72% and 1.47% respectively, for the method by hand and 1.10, 1.54% and 0.27, 0.38% respectively, for the IAS methods. It is concluded that the IAS method is an objective and accurate alternative for reading the agar diffusion test.