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Specific binding of dl‐cloprostenol and d‐cloprostenol to PG F 2α receptors in bovine corpus luteum and myometrial cell membranes
Author(s) -
RE G.,
BADINO P.,
NOVELLI A.,
VALLISNERI A.,
GIRARDI C.
Publication year - 1994
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1994.tb00277.x
Subject(s) - corpus luteum , cloprostenol , endocrinology , prostaglandin , receptor , medicine , chemistry , membrane , biology , ovary , hormone , biochemistry , ovulation
Prostaglandin F 2α receptors (PGF 2α Rs) were measured in bovine corpus luteum and myometrial cell membranes using a radiometric method. The inhibition of labelled PGF 2α binding exerted by d‐cloprostenol, dl‐cloprostenol, PGF 2α and PGE 1 (l0– 11 M to 10 –4 M) was evaluated in vitro. Results strongly suggest that cloprostenol binding to PGF 2α Rs is stereospecific. d‐Cloprostenol and PGF 2α were equipotent, about 150 times more potent than d‐cloprostenol (P < 0.05) and approximately 280 times more potent than PGE, (P < 0.05) in inhibiting [3H]PGF 2α binding to corpus luteum cell membranes. Such differences were less evident in myometrial cell membranes, where d‐cloprostenol and PGF 2α were about 10 times more potent than d‐cloprostenol (P < 0.05) and approximately 95 times more potent than PGE l (P < 0.05).