Premium
Effects of age and indomethacin on response and sensitivity of pulmonary artery to phenylephrine and to histamine in pigs
Author(s) -
GUSTIN P.,
ANSAY M.,
ADVENIER C.
Publication year - 1993
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1993.tb00165.x
Subject(s) - phenylephrine , histamine , medicine , endocrinology , chemistry , agonist , arachidonic acid , potency , anesthesia , biochemistry , receptor , blood pressure , in vitro , enzyme
The vasoconstrictor effects of phenylephrine and histamine were investigated in isolated strips of pulmonary arteries in pigs during ageing. Interactions between phenylephrine‐induced responses and arachidonic acid derivatives were also studied by incubating the blood‐vessels with indomethacin. Potency (pD2 values) and maximal effects (E max x) recorded in 5‐week‐old piglets (group I, n = 5) with phenylephrine [5.71 ± 0.17 and 0.76 ± 0.22 g/mg of dry tissue respectively (mean ± SEM)] were similar to values found in 12‐week‐old animals (group 2, n = 5) (5.49 ± 0.30 and 1.06 ± 0.27 g/mg of dry tissue respectively). The sensitivity and responsiveness of tissues to this agonist were significantly reduced in 26‐week‐old mature pigs (group 3, n = 6) as indicated by the decrease in pD2 (3.91 ± 0.23; P < 0.01) and E max (0.27 ± 0.13 g/mg of dry tissue; P < 0.05) values observed in this group. Histamine (10 _3 M)‐induced maximal responses (E max ) were significantly higher in group 2 (2.23 ± 0.49 g/mg) than in group 1 (0.85 ± 0.11 g/mg; P < 0.05) and in group 3 (0.48 ± 0.10 g/mg; P < 0.01). In 5‐week‐old animals, indomethacin (3.10˜ 5 M) significantly ( P < 0.05) shifted the concentration‐response curve to phenylephrine to the right (0.28 log. units) and depressed contractions to this drug as shown by the significant decrease of 39.5% ( P < 0.05) in E max . This cyclo‐oxygenase inhibitor had no effect in other groups. These data indicate that phenylephrine is a potent and effective vasoconstrictor agent for the main pulmonary arteries in 5‐week‐old piglets and that alpha‐1‐adrenergic‐induced contractions are enhanced by cyclo‐oxygenase products. These findings can be related with the high reactivity of pulmonary vascular smooth muscles in these animals.