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Quantification of percutaneous absorption of metronidazole and levamisole in the fire‐bellied toad ( Bombina orientalis )
Author(s) -
MOMBARG M.,
CLAESSEN H.,
LAMBRECHTS L.,
ZWART P.
Publication year - 1992
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1992.tb01035.x
Subject(s) - levamisole , metronidazole , dose , chemistry , high performance liquid chromatography , chromatography , absorption (acoustics) , urine , toad , pharmacology , antibiotics , biology , medicine , biochemistry , materials science , immunology , composite material
Spot‐on application has proved to be an effective way to reach therapeutic doses of metronidazole and levamisole in fire‐bellied toads. The percutaneous absorption of metronidazole and levamisole was quantified, using an aqueous solution of 1.008 mg/ml of metronidazole and an aqueous solution of 3.767 mg/ ml of levamisole. High Performance Liquid Chromatography (HPLC) analysis of the percutaneous absorption revealed that of the amount applied, 75% of metronidazole and 90% of levarnisole was absorbed. This resulted during 3 days of application in dosages of 23 mg/kg BW of metronidazole and 94 mg/kg BW of levamisole. Of the absorbed substances, 48% of metronidazole and 9% of levamisole were excreted in urine and faeces as unmetabolised substances.