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Parainfluenza‐3 virus‐induced enhancement of histamine release from calf lung mast cells – effect of levamisole
Author(s) -
OGUNBIYI P. O.,
BLACK W. D.,
EYRE P.
Publication year - 1988
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1988.tb00193.x
Subject(s) - histamine , levamisole , chemistry , mast (botany) , mast cell , pharmacology , virus , lung , virology , biology , immunology , medicine
Ogunbiyi, P.O., Black, W.D. & Eyre, P. Parainfluenza‐3 virus‐induced enhancement of histamine release from calf lung mast cells – effect of levamisole. J. vet. Pharmacol. Therap. 11 , 338–344. Clinically healthy calves were divided into five groups. Group 1 served as control; Group 2 received levamisole (LEV), 3 mg/kg, s.c; Group 3 was aerosolized with parainfluenza‐3 virus (PI‐3); Group 4 received LEV and PI‐3 and Group 5 was inoculated with Pasteurella haemolytica. They were killed 6 days after virus exposure or 5–6 days after bacterial inoculation. Lung mast cells were prepared by enzymatic treatment. Mast cell histamine (HIST) release was assayed spectro‐fluorometrically. Total HIST (m̈g/g) in mast cells was as follows (x̄± SEM): control (5.30 ± 0.26); LEV (5.27 ± 0.31); PI‐3 (6.37 ± 0.65); LEV ± Pl‐3 (6.21 ± 0.51); P. haemolytica (7.06 ± 0.85). Spontaneous HIST release was as follows (% total, x̄± SEM): control (10.38 ± 1.09), LEV (11.95 ± 2.13), PI‐3v (73.57 ± 11.97), PI‐3v ± LEV (19.50 ± 3.03), and P. haemolytica (70.59 ± 5.94). Calcium ionophore A23187 (5 times 10 ‐6 M) ‐induced release (% total, x̄± SEM) was: 51.53 ± 3.05, 50.02 ± 2.70, 83.91 ± 4.09, 75.21 ± 4.51 and 70.59 ± 6.91 for control, LEV, PI‐3, LEV ± PI‐3 and P. haemolytica groups, respectively. Both virus and bacteria increased HIST content of lung mast cells and enhanced ionophore‐induced release. Levamisole significantly reduced spontaneous HIST release in virus‐infected calves but had no effect on ionophore‐induced release. Results suggest a significant role for HIST in pathogenesis of bovine microbial pneumonia and that LEV probably does not modulate non‐immunologic release of HIST from bovine lungs.