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Zearanol metabolism by subcellular fractions from lamb liver
Author(s) -
POMPA G.,
MONTESISSA C.,
LAURO F. M. DI,
FADINI L.,
CAPUA C.
Publication year - 1988
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1988.tb00141.x
Subject(s) - nad+ kinase , metabolite , microsome , incubation , metabolism , chemistry , nicotinamide , cytosol , biochemistry , nicotinamide adenine dinucleotide , epimer , chromatography , in vitro , enzyme , stereochemistry
Pompa, G., Montesissa, C., Di Lauro, F.M., Fadini, L. & Capua, C. Zearanol metabolism by subcellular fractions from lamb liver.J, vet. Pharmacol. Therap. 11, 197–203. Zearanol (7‐α‐zearalanol or α‐ZAL) metabolism in vitro by subcellular fractions (microsome and cytosol) from lamb livers was investigated. The use of a high performance liquid chromatography (HPLC) technique capable of resolving epimers, revealed that when nicotinamide adenine dinucleotide (NAD) was added as a co‐factor to metabolic mixtures, the oxidized form, zearalanone (ZAN) was the major metabolite, although a small amount of 7‐β‐zearalanol (β‐ZAL) was also produced. In order to confirm β‐ZAL as a product of ZAN reduction, the metabolism of the latter, by microsome and cytosol in the presence of NADH as co‐factor, was investigated. The results obtained revealed that both α‐ZAL and β‐ZAL were present at the end of the incubation, the former at a higher concentration than the latter. When NAD and NADH were added as co‐factors to incubation mixtures containing α‐ZAL, the production of β‐ZAL was increased as a consequence of the higher ZAN reduction in the presence of the reducing co‐factor. Nevertheless, ZAN remained the major metabolite produced from α‐ZAL by both the subcellular fractions investigated.