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An improved radioimmunoassay for the anabolic agent hexoestrol, using a monoclonal antibody
Author(s) -
CARTER A. P. D.,
COTTINGHAM J. D.,
DIXON S. N.,
HEITZMAN R.J.
Publication year - 1985
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1985.tb00968.x
Subject(s) - polyclonal antibodies , monoclonal antibody , radioimmunoassay , chemistry , antibody , urine , chromatography , detection limit , monoclonal , anabolism , microbiology and biotechnology , biochemistry , biology , immunology
A monoclonal antibody was raised against hexoestrol coupled to bovine serum albumin. The antibody cross‐reacted with the stilbenes, diethylstilboestrol (10%) and dienoestrol (4%), but had no cross‐reaction (<0.01%) with other anabolic agents. A radioimmunoassay method using the monoclonal antibody has been validated and used to measure residues of hexoestrol in the urine of treated cattle. The limit of detection was 0.6 pg/ml urine at the 95% confidence limit. The results were compared with those obtained using polyclonal antibodies. Although there was a good correlation between the results, the use of monoclonal antibody gave more reliable results than those obtained with available polyclonal antibodies. The monoclonal antibody, because of its quality and theoretically limidess supply, is very suitable for use in large scale screening or monitoring programmes for regulating the use of hexoestrol.