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Interaction of Fasciola hepatica with albendazole and its metabolites
Author(s) -
FETTERER R. H.,
REW R. S.
Publication year - 1984
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1984.tb00886.x
Subject(s) - fasciola hepatica , albendazole , incubation , anthelmintic , in vitro , triclabendazole , chemistry , feces , liver fluke , biology , helminths , chromatography , microbiology and biotechnology , biochemistry , immunology , ecology
Adult Fasciola hepatica recovered from sheep 12 and 24 h after a single oral dose of albendazole (20 mg/kg) contained significant amounts of two oxidized metabolites of albendazole (ABZ), a sulphoxide (SX) and a sulphone (SO), but not ABZ. Flukes incubated in vitro with 10 μM SX or SO contained these metabolites at a level two to three times the level observed in flukes recovered from sheep 24 h after a curative oral dose of ABZ. The concentration of ABZ in flukes was 10‐fold greater than either SX or SO after a 24 h in vitro incubation in 10 μM of the respective drug. Flukes exposed to ABZ in vitro contained twofold higher SX levels than SX‐treated flukes due to a combination of spontaneous oxidation in media and fluke‐mediated oxidation of ABZ. Measurement of end‐products of glucose metabolism following 24 h incubation in 10 μM of either ABZ, SX or SO did not show a significant difference between treated and untreated flukes.