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The recombinant DNA technology
Author(s) -
TIEMEIER DAVID C.
Publication year - 1983
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1983.tb00449.x
Subject(s) - recombinant dna , dna , in vitro recombination , cloning (programming) , molecular cloning , computational biology , biology , fragment (logic) , gene , dna replication , microbiology and biotechnology , genetics , chemistry , computer science , complementary dna , algorithm , programming language
The recombinant DNA technology or DNA cloning permits the isolation, amplification, and precise manipulation of specific DNA fragments. This is generally accomplished by linking or recombining the desired DNA fragment with a DNA molecule, termed the vector, which is capable of directing the replication of itself in a suitable host cell and any DNA segment covalently attached to it. Using this and associated technologies, it is possible to produce large amounts of specific proteins and to modify cell types by introducing the genes for proteins that are otherwise absent. Moreover, it is now possible to construct variants of naturally‐occurring proteins with improved biological or physical properties.