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Radioimmunoassay of the anabolic agent zeranol I. Preparation and properties of a specific antibody to zeranol
Author(s) -
DIXON S. N.
Publication year - 1980
Publication title -
journal of veterinary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.527
H-Index - 60
eISSN - 1365-2885
pISSN - 0140-7783
DOI - 10.1111/j.1365-2885.1980.tb00424.x
Subject(s) - zeranol , antiserum , radioimmunoassay , chemistry , metabolite , antibody , bovine serum albumin , chromatography , biochemistry , endocrinology , biology , immunology
A highly specific antibody has been raised in sheep to an antigen prepared by coupling zeranol hemisuccinate to bovine serum albumin. An assay procedure is described and has been used to measure the cross‐reactivity of the antiserum, and evaluate the sensitivity of the assay method. The only compounds which reacted with the antibody were zeranol 100%, zearalanone 100%, zearalenone 19% and zearalenol 19%. The sensitivity of the assay procedure allows the determination of zeranol and/or its main metabolite zearalanone between 100 and 1000 pg.