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Cytotoxicity of one‐step dentin‐bonding agents toward dental pulp and odontoblast‐like cells
Author(s) -
YASUDA Y.,
INUYAMA H.,
MAEDA H.,
AKAMINE A.,
NÖR J. E.,
SAITO T.
Publication year - 2008
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.2008.01885.x
Subject(s) - cytotoxicity , dentin , trypan blue , pulp (tooth) , odontoblast , chemistry , viability assay , microbiology and biotechnology , nuclear chemistry , dentistry , biochemistry , cell , in vitro , medicine , biology
Summary The purpose of this study was to compare the cytotoxicity of five one‐step dentin‐bonding agents on human dental pulp and odontoblast‐like cells (MDPC‐23). Photopolymerized and unpolymerized samples of these dentin‐bonding agents were prepared and incubated with dental pulp or MDPC‐23 cells. After 24 or 72 h of incubation, the number of unstained cells with trypan blue was counted. The staining of cells with trypan blue stands for a cytotoxicity. The pulp cell and MDPC‐23 cytotoxicity of polymerized sample treatment increased in the order of AQ Bond Plus (AQ)