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Genotoxicity evaluation of five different dentin bonding agents by chromosomal aberration analysis
Author(s) -
PRICA D.,
GALIĆ N.,
ŽELJEŽIĆ D.,
PRICA A.
Publication year - 2006
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.2006.01606.x
Subject(s) - genotoxicity , dentin , adhesive , serial dilution , dentin bonding agents , elution , bond strength , dentistry , chemistry , chromatography , materials science , composite material , medicine , toxicity , pathology , organic chemistry , alternative medicine , layer (electronics)
summary Dentin bonding agents became unavoidable in today's aesthetic restorative dentistry. Nevertheless, more and more evidences on their possible cytotoxity and/or genotoxicity emerge. Still, only limited number of studies has been published on that issue. In our work we evaluated possible genotoxicity of five different adhesives: Adper TR Single Bond, Adper TR Single Bond 2 with nanofiller, Excite ® , OptiBond ® Solo Plus TR and Prompt TR L‐pop. Genotoxicity assessment was carried out on human lymphocytes in vitro , using chromosomal aberration analysis. Polymerized adhesives were tested at three different dilutions of the 0·5 g mL −1 eluate stock (2·5 × 1:10 6 , 1:10 6 and 1:10 5 ) after 1 h, 24 h and 5 days of elution. Slight but significant increase in the number of chromatid breaks was observed after 24‐h elution period, for adhesives Adper TR Single Bond 2, Excite ® , and OptiBond ® Solo Plus TR at dilutions of 1:10 6 and 1:10 5 , and for other two only at dilution of 1:10 5 . First three adhesives also appeared to be slightly genotoxic after 1 h of elution but only at 1:10 5 . As a bonding agent remains in close contact with living dental tissue over a long period of time, information on their possible genotoxicity and carcinogenicity should be more clearly clarified in the near future.