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In vivo evaluation of the biocompatibility of three current bonding agents
Author(s) -
TEIXEIRA H. M.,
DO NASCIMENTO A. B. L.,
HEBLING J.,
DE SOUZA COSTA C. A.
Publication year - 2006
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.2005.01591.x
Subject(s) - calcium hydroxide , biocompatibility , connective tissue , fixative , in vivo , capsule , chemistry , materials science , biomedical engineering , inflammatory response , calcium , dentistry , pathology , medicine , staining , inflammation , biology , organic chemistry , immunology , botany , microbiology and biotechnology
summary The aim of this in vivo study was to evaluate the biocompatibility of three current bonding agents and calcium hydroxide cement. Sixty polyethylene tubes filled with the following materials: Group 1: Prime & Bond NT (PB – Dentsply, US; Group 2: Bond 1 (BO – Jeneric/Pentron, US); Group 3: Optibond Solo (OP – Kerr, US); and Group 4 (control): calcium hydroxide cement – Dycal (CH – Dentsply, US) were implanted into the connective tissue of 30 rats. After 15, 30 and 60 days, the implants were excised and the animals sacrificed. The biopsies were immersed in Karnovsky (pH, 7.2) fixative solution for 48 hours, and processed using routine histological technique. Six‐micron‐thick sections were cut and stained with hematoxilin and eosin and Masson's trichome technique. Microscopic evaluation was used to compare the connective tissue reactions caused by the experimental and control materials adjacent to the tube opening. At 15 days, the experimental and control materials triggered a moderate to intense inflammatory response which gave rise to a thick capsule adjacent to the tube opening. With time, the inflammatory reaction decreased. At 60 days, the connective tissue adjacent to the bonding agents exhibited a persistent inflammatory response mediated by macrophages and giant cells which were engulfing displaced resin components. On the other hand, for the control group (calcium hydroxide) no inflammatory response associated with a thin capsule adjacent to the material was observed even at the 30‐day period. The hard‐setting calcium hydroxide cement allowed complete healing and was considered more biocompatible than the bonding agents.