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Comparative radical production and cytotoxicity induced by camphorquinone and 9‐fluorenone against human pulp fibroblasts
Author(s) -
Atsumi T.,
Ishihara M.,
Kadoma Y.,
Tonosaki K.,
Fujisawa S.
Publication year - 2004
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.2004.01357.x
Subject(s) - cytotoxicity , chemistry , photosensitizer , reactive oxygen species , polyhydroxybutyrate , methacrylate , liposome , photochemistry , polymerization , nuclear chemistry , organic chemistry , biochemistry , polymer , in vitro , biology , bacteria , genetics
summary Camphorquinone (CQ) is widely used as a photo‐initiator in dental materials; however, its cytotoxicity against human pulp fibroblasts (HPF) and particularly the effects of 2‐dimethylaminoethyl methacrylate (DMA), a reducing agent and visible light (VL) irradiation on it remain unknown. So we investigated the cytotoxic and reactive oxygen species (ROS)‐producing effects of CQ with or without DMA, in the presence or absence of VL on HPF cells. The free‐radical production activity of CQ was measured by two different methods [using diphenylpicryl hydrazyl and galvinoxyl]. The phase‐transition properties of dipalmitoylphosphatidyl choline (DPPC) liposomes, as a model for biomembranes, induced by CQ were investigated by differential scanning calorimetry. These findings were compared with those of 9‐fluorenone (9F), an aromatic photo‐initiator with long conjugated groups. Camphorquinone with VL irradiation increased the radical production, whereas 9F with VL irradiation increased ROS production, as well as effecting changes in the DPPC phase‐transition properties. The cytotoxicity of CQ towards HPF cells was smaller than that of 9F despite greater radical production. The addition of DMA to the photosensitizer enhanced the free‐radical production without increasing the ROS level or the cytotoxicity. Camphorquinone/DMA is a valuable combination for the polymerization of dental resins.