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In vitro cytotoxicity of six dentin bonding agents
Author(s) -
KoliniotouKoubia E.,
Dionysopoulos P.,
Koulaouzidou E. A.,
Kortsaris A. H.,
Papadogiannis Y.
Publication year - 2001
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.2001.00769.x
Subject(s) - cytotoxicity , dentin , dentin bonding agents , chemistry , nuclear chemistry , dentistry , in vitro , materials science , bond strength , composite material , adhesive , medicine , biochemistry , layer (electronics)
The cytotoxicity of six dentin bonding agents (Syntac, Solobond, Bond 1, Scotchbond 1, Heliobond and F‐2000) was tested against an established cell line, L929. Under aseptic conditions 3, 5 and 10 μL dentin bonding agents were placed in the centre of Petri dishes. Each dish was covered with a 5‐mL suspension of fibroblasts at a concentration of 40 000 cells mL –1 . The cultures were incubated at 37 °C and cytotoxicity was assessed by a quantitative technique at 24 and 72 h. All the dentin bonding agents were found to be cytotoxic. Scotchbond 1 and F‐2000 showed the highest cytotoxicity followed by Solobond and Bond 1. Heliobond and Syntac were the least toxic materials.