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Studies on cytotoxicity of nickel ions using C3H10T1/2 fibroblast cells
Author(s) -
Taira M.,
Toguchi M. S.,
Hamada Y.,
Okazaki M.,
Takahashi J.,
Ito R.,
Toyosawa S.,
Ijyuin N.
Publication year - 2000
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.2000.00608.x
Subject(s) - cytotoxicity , fibroblast , dissolution , nuclear chemistry , nickel , metal ions in aqueous solution , ion , chemistry , metal , cell , in vitro , biochemistry , organic chemistry
Cytotoxicity of Ni ions were examined using C3H mouse derived 10T1/2 fibroblast cells. It became evident that Ni ions had dose‐dependent cytotoxicity. The cell number incubated in DME medium containing 0·04 m m /L Ni ion for 6 days was reduced to half that in control DME medium without Ni. The cell totally disappeared in DME medium containing 2 m m /L Ni ion. The dissolution of Ni ions from Ni‐containing metallic restorations must be lower than these concentration levels so that oral tissues might not be damaged. No neoplastic transformation was found on all cells examined.