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Effect of polylactic acid on osteoinduction of demineralized bone: preliminary study of the usefulness of polylactic acid as a carrier of bone morphogenetic protein
Author(s) -
SAITOH H.,
TAKATA T.,
NIKAI H.,
SHINTANI H.,
HYON S.H.,
IKADA Y.
Publication year - 1994
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.1994.tb01157.x
Subject(s) - polylactic acid , bone morphogenetic protein , bone formation , cartilage , chemistry , bone morphogenetic protein 2 , biomedical engineering , dentistry , anatomy , polymer , medicine , in vitro , biochemistry , organic chemistry , gene
Summary To evaluate the usefulness of polylactic acid (PLA), a bioabsorbable and plastic polymer, as a carrier of bone morphogenetic protein (BMP), a preliminary study has been carried out to investigate any negative effect of PLA on osteoinduction of demineralized bone (DB). PLA (10,600mol. wt) was mixed with DB particles (1– 1.5 mm square) prepared from rat femurs and laid subcutaneously on the intercostal muscle of 4‐week‐old Wistar rats. The PLA/DB pellets were harvested at 2, 4, 8 and 24 weeks after the operation, and prepared for light microscopic examination. Histological examination revealed cartilage formation at 2 weeks and new bone formation at 4 weeks. Extensive bone and marrow formation were observed at 24 weeks. PLA was gradually absorbed and completely disappeared at 24 weeks to be replaced by connective tissue. These results demonstrate that PLA does not have a negative influence on the osteoinductive activity and that PLA could well be a promising bioabsorbable carrier of BMP.