Premium
A method for the estimation of traces of chlorhexidine in water
Author(s) -
WILSON S.J.,
WILSON H.J.
Publication year - 1993
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.1993.tb01604.x
Subject(s) - bromothymol blue , chlorhexidine , chemistry , extraction (chemistry) , carbon tetrachloride , chromatography , dentistry , organic chemistry , medicine
A simple method of estimating traces of chlorhexidine in water using bromothymol blue is described. The procedure involves the extraction of the chlorhexidine/bromothymol blue complex into carbon tetrachloride from a buffered neutral solution and measurement of the absorbances of the extracts in a spectrophotometer. The extracted complex has good colour stability, and the method is accurate, reproducible, and very sensitive, with concentrations of chlorhexidine down to 0.05 μg ml ‐1 measurable.