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In vitro toxicity tests of zinc phosphate cement
Author(s) -
TYAS M. J.
Publication year - 1978
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.1978.tb01252.x
Subject(s) - acid phosphatase , membrane , chemistry , phosphate , dehydrogenase , succinic acid , biochemistry , in vivo , lactate dehydrogenase , zinc , nuclear chemistry , enzyme , biology , organic chemistry , microbiology and biotechnology
Summary BHK‐21 (C‐13) cells were exposed to freshly mixed zinc phosphate cement for 0·5, 1 and 2 h in a simulated cavity apparatus. The effect on the cells was evaluated by quantitative measurement of acid phosphatase and succinic dehydrogenase reaction product in order to determine organelle membrane permeability. After 0·5 h cellmaterial contact there was no significant effect. After 1 h cell‐material contact, there was slight labilization of the lysosomal membranes, and a significant decrease in succinic dehydrogenase activity. The mean overall stain density in the experimental cells was 109% and 57% of the control values for acid phosphotase and succinic dehydrogenase respectively. After 2 h cell‐material contact, there was significant labilization of the lysosomal membranes, and also labilization of the mitochondrial membranes. The mean overall stain densities were 114 % and 75 % of the control values for acid phosphatase and succinic dehydrogenase respectively. The relevance of these findings to the in vivo situation is discussed.