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Cytotoxicity of temporary crown and bridge materials
Author(s) -
DAHL BJøRN L.,
øRSTAVIK JON
Publication year - 1976
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/j.1365-2842.1976.tb01448.x
Subject(s) - cytotoxicity , trypan blue , in vitro , chemistry , in vivo , dentistry , biochemistry , medicine , biology , microbiology and biotechnology
Summary In vitro cytotoxicity of freshly prepared temporary crown and bridge materials was studied using mouse fibroblasts prelabelled with 3 H‐thymidine grown in shake flask cultures. Cytotoxicity was measured as (1) lack of cell multiplication, (2) stainability with Trypan blue and (3) release of 3 H‐labelled material to the culture medium. All the materials tested (zinc oxide‐eugenol cement (ZOE), Scutan® and Sevriton®) were cytotoxic in this system, ZOE most strongly. This finding favours the view that the in vitro cytotoxicity of dental materials should not be used alone as a screening test for their biological compatibility under clinical conditions since clinical experience and previous in vivo findings have established that ZOE is biologically well tolerated by the dental tissues.