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Role of Oestrogen Receptors on the Modulation of NADPH‐Diaphorase‐Positive Cell Number in Supraoptic and Paraventricular Nuclei of Ovariectomised Female Rats
Author(s) -
Grassi D.,
Lagunas N.,
Amorim M.,
Pinos H.,
Panzica G.,
GarciaSegura L. M.,
Collado P.
Publication year - 2013
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.2012.02387.x
Subject(s) - medicine , endocrinology , chemistry , agonist , supraoptic nucleus , antagonist , nitric oxide , receptor , hypothalamus , nadph oxidase , biology , oxidative stress
Modulation of the nitric oxide producing system (demonstrated via the NADPH ‐diaphorase histochemical reaction) by oestradiol has been established in several structures of the rat brain. The present study aimed to explore the possible regulation of NADPH ‐diaphorase activity by oestradiol in neurones of the supraoptic ( SON ) and paraventricular ( PVN ) nuclei and the role of oestrogen receptors ( ER α and ER β) in this regulation. Adult ovariectomised rats were divided into six groups and injected either with vehicle or a single dose of oestradiol, a selective ER α agonist‐ PPT [4,4′,4″‐(4‐propyl‐[1H]‐pyrazole‐1,3,5‐triyl)trisphenol] , a selective ER β agonist‐DPN [2,3‐bis(4‐hydroxyphenyl)‐propionitrile], a selective ER α antagonist‐ MPP [1,3‐bis(4‐hydroxyphenyl)‐4‐methyl‐5‐[4‐(2‐piperidinylethoxy)phenol]‐1H‐pyrazole dihydrochloride] or a selective ER β antagonist‐ PHTPP (4‐[2‐phenyl‐5,7‐bis(trifluoromethyl)pyrazolo[1,5‐a]pyrimidin‐3‐yl]phenol) . The number of NADPH ‐diaphorase positive elements in the SON and the PVN was modulated by both ER s but, depending on the nucleus, ER α and ER β ligands induced different effects. These results suggest that the regulation of nitrergic system by ER s may play a role in the control of oestrogen‐dependent physiological mechanisms regulated by the SON and the PVN .