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Selective Up‐Regulation of J un D Transcript and Protein Expression in Vasopressinergic Supraoptic Nucleus Neurones in Water‐Deprived Rats
Author(s) -
Yao S. T.,
Gouraud S. S.,
Qiu J.,
Cunningham J. T.,
Paton J. F. R.,
Murphy D.
Publication year - 2012
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.2012.02362.x
Subject(s) - supraoptic nucleus , microbiology and biotechnology , messenger rna , biology , vasopressin , western blot , gene expression , immunohistochemistry , reverse transcription polymerase chain reaction , transcription (linguistics) , transcription factor , endocrinology , medicine , chemistry , gene , biochemistry , immunology , linguistics , philosophy
The magnocellular neurones ( MCN ) of the supraoptic nucleus ( SON ) undergo reversible changes during dehydration. We hypothesise that alterations in steady‐state transcript levels might be partially responsible for this plasticity. In turn, regulation of transcript abundance might be mediated by transcription factors. We have previously used microarrays to identify changes in the expression of m RNA s encoding transcription factors in response to water deprivation. We observed down‐regulation of 11 and up‐regulation of 31 transcription factor transcripts, including members of the activator protein ‐1 gene family, namely c‐fos , c‐jun , fosl1 and junD . Because J un D expression and regulation within the SON has not been previously described, we have used in situ hybridisation and the quantitative reverse transcriptase ‐ polymerase chain reaction to confirm the array results, demonstrating a significant increase in J un D mRNA levels following 24 and 72 h of water deprivation. Western blot and immunohistochemistry revealed a significant increase in J un D protein expression following dehydration. Double‐staining fluorescence immunohistochemistry with a neurone‐specific marker ( N eu N ) demonstrated that J un D staining is predominantly neuronal. Additionally, J un D immunoreactivity is observed primarily in vasopressin‐containing neurones with markedly less staining seen in oxytocin‐containing MCN s. Furthermore, J un D is highly co‐expressed with c‐ F os in MCN s of the SON following dehydration. These results suggest that J un D plays a role in the regulation of gene expression within MCN s of the SON in association with other F os and J un family members.