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Peripheral Lipopolysaccharide Administration Induces Cytokine mRNA Expression in the Viscera and Brain of Fever‐Refractory Mice Lacking Microsomal Prostaglandin E Synthase‐1
Author(s) -
Nilsberth C.,
Hamzic N.,
Norell M.,
Blomqvist A.
Publication year - 2009
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.2009.01888.x
Subject(s) - endocrinology , medicine , lipopolysaccharide , prostaglandin , cytokine , tumor necrosis factor alpha , prostaglandin e2 , biology , interleukin , prostaglandin e , chemistry
We examined the expression of interleukin (IL)‐1β, IL‐6 and tumour necrosis factor (TNF) α in mice lacking microsomal prostaglandin E synthase‐1 (mPGES‐1), which neither produce prostaglandin E 2 , nor mount a febrile response upon immune challenge. Intraperitoneal lipopolysaccharide (LPS) injection resulted in a strongly induced expression of all three cytokines in the brain and viscera, similar to wild‐type animals. Several brain regions additionally showed modest induction of receptors for these cytokines in both genotypes. Telemetric recordings of body temperature showed that the mPGES‐1 deficient mice remained afebrile upon LPS challenge, in contrast to the prominent fever displayed by the wild‐type mice. These data demonstrate that LPS‐induced cytokine expression occurs independently of prostaglandin E 2 , and imply that endogenously expressed IL‐1β, IL‐6, and TNFα are not pyrogenic per se , supporting the role of prostaglandin E 2 as the final and obligatory mediator of LPS‐induced fever.