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Male and Female Prolactin Receptor mRNA Expression in the Brain of a Biparental and a Uniparental Hamster, Phodopus , Before and After the Birth of a Litter
Author(s) -
Ma E.,
Lau J.,
Grattan D. R.,
Lovejoy D. A.,
WynneEdwards K. E.
Publication year - 2005
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.2005.01278.x
Subject(s) - phodopus , medicine , endocrinology , biology , prolactin , prolactin receptor , hamster , messenger rna , lactation , hormone , genetics , gene , pregnancy
Prolactin receptor (PRL‐R) mRNA transcript level was quantified in the choroid plexus (ChP) of a naturally biparental hamster, Phodopus campbelli , and its otherwise similar, yet nonpaternal, sibling species, Phodopus sungorus . Pair‐housed males and females on the day before the birth of their first litter (G17), the day after birth (L1), lactation day 5 (L5), and unpaired animals that were sexually naïve, were tested. PRL‐R mRNA transcript level relative to total RNA, was evaluated by reverse transcriptase‐polymerase chain reaction using primers common to the long‐ and short‐form of the PRL‐R in Phodopus . In the ChP, a region implicated in prolactin transport into the central nervous system, females had the expected increase in PRL‐R mRNA transcript from dioestrus to L5, consistent with known actions of prolactin. As predicted, males and females of the biparental species were similar, although PRL‐R mRNA in naïve males was higher than in dioestrus females. Males of the two species also differed as predicted. PRL‐R mRNA transcript levels were higher in the biparental males . In addition, P. campbelli males had low PRL‐R mRNA at G17 compared to L5. By contrast, non‐paternal P. sungorus males had elevated PRL‐R mRNA transcript levels on G17 relative to unpaired males. We conclude that PRL‐R mRNA in the ChP is differentially regulated before and after birth in a paternal and a nonpaternal male.