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Effect of Parathyroid Hormone and Calcitonin on Cholinergic Markers in Rat Parathyroid Gland
Author(s) -
Stern Javier E.,
Cardinali Daniel P.
Publication year - 1995
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/j.1365-2826.1995.tb00810.x
Subject(s) - medicine , endocrinology , parathyroid hormone , calcitonin , chemistry , choline , acetylcholine , cholinergic , antagonist , biology , receptor , calcium
The effect of parathyroid hormone (PTH) and calcitonin on 3 H‐choline uptake and on 3 H‐acetylcholine synthesis by rat parathyroid glands were examined. Incubation of tissue for 120 min with PTH or calcitonin resulted in an inverted bell‐shaped, dose‐dependent inhibition of 3 H‐choline uptake, with a maximal effect at 10 −9 M Concentration. The effect of PTH on parathyroid choline uptake was blunted by preincubation with the PTH antagonist NLe 8–18 ‐PTH (3–34) amide. PTH brought about a dose‐dependent inhibition of 3 H‐choline conversion to 3 H‐acetylcholine, with significant effects at 10 −8 M concentration or higher. This inhibitory effect of PTH on 3 H‐acetylcholine synthesis was blocked by co‐incubation with the PTH antagonist NLe 8–18 ‐PTH (3–34) amide. Only at a 10 −7 M concentration calcitonin was effective to impair the in vitro conversion of radioactive choline into 3 H‐acetylcholine by parathyroid fragments. The results indicate that, in vitro , PTH, and to a less extent, calcitonin, inhibit cholinergic activity in rat parathyroid glands.